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References External links Category:Windows softwareMiniaturized affinity sensor by incorporating electropolymerized DNA probes and non-equilibrium surface-transfer ruthenium electrochemiluminescence amplification. Herein, the electropolymerized poly(dA)/poly(dT)-modified electrode was successfully fabricated as a non-equilibrium electrode for the amplification of the ruthenium(II)-tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) electrochemiluminescence (ECL) signal. Ru(bpy)(3)(2+) was incorporated into a stable DNA duplex (poly(dA)/poly(dT)) by electrostatic interaction, with a loading of 20 μM Ru(bpy)(3)(2+) in the poly(dA)/poly(dT). The poly(dA)/poly(dT)-modified electrode was prepared by electrochemical polymerization of N,N'-dimethylbenzidine (DMB) in the presence of the poly(dA)/poly(dT) and used for the detection of prostate specific antigen (PSA). The results show that the modified electrode exhibits a linear response to PSA in the concentration range of 10-200 pg·mL(-1). The detection limit was 7.5 pg·mL(-1) and the relative standard deviation (RSD) was 6.8% for 10 pg·mL(-1) PSA. This electropolymerized poly(dA)/poly(dT)-modified electrode was further used for the detection of human alpha-fetoprotein (AFP). The linear response range was 1-300 ng·mL(-1) with a detection limit of 0.65 ng·mL(-1). Moreover, the electrochemical polymerization of DMB could be used for preparing highly sensitive DNA probes for the detection of microorganisms by ECL. The poly(dA)/poly(dT)-modified electrode was used for detecting the gene of the common pathogenic bacteria E. coli in complex biofilm matrix. This study demonstrated that the electropolymerized poly(dA)/poly(dT) probe was a sensitive and selective platform for nucleic acid detection. are similar to the ones described above. Releasing a successful fan-funded Kickstarter isn’t the end of your campaign. You may









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Hiren Mini Xp Iso |VERIFIED| Download

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